myd88 signaling regulation cells Search Results


human myd88 antibody  (Bio-Techne corporation)
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Bio-Techne corporation human myd88 antibody
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myd88 signaling regulation cells  (Addgene inc)
93
Addgene inc myd88 signaling regulation cells
Fig. 1 MyD88D162E mutant mice are protected from DSS-induced acute colitis. Schematic presentation of the DSS-induced colitis experiment. To induce acute colitis, mice were drinking tap water, supplemented with 2% DSS for 8 consecutive days ad libitum. A Disease activity index was defined by observing the stool consistency and the presence of the gross or occult blood. The weight loss was calculated from the weight difference on day1 and day 8 and the colon length was measured from the body of the caecum to the distal part of the rectum. B The part of the colon was washed and IL-6 cytokine was measured using ELISA assay. C Blood was collected and IL-6 was detected in serum using ELISA. D-F Il6, Il1b, and <t>MyD88</t> mRNA expression was detected using qPCR in colon tissue homogenate. G <t>MyD88</t> <t>protein</t> detection from obtained colon tissue of treated mice, using Western blot. β-actin was used as loading control. H Schematic presentation of the experiment. LPS was i.p. injected. After 4 h IL-6 and IL-10 cytokines were analyzed in the serum. Combined data from one (B, C. H) or two (A, D-F) indep. exp. are shown as mean ± SEM. LN transformation was applied for the data used in statistical tests (D-F). Studentʾs unpaired t-test was used. p values of < 0.05 (*), < 0.01 (**) are indicated
Myd88 Signaling Regulation Cells, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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kinases erk  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology kinases erk
Fig. 1 MyD88D162E mutant mice are protected from DSS-induced acute colitis. Schematic presentation of the DSS-induced colitis experiment. To induce acute colitis, mice were drinking tap water, supplemented with 2% DSS for 8 consecutive days ad libitum. A Disease activity index was defined by observing the stool consistency and the presence of the gross or occult blood. The weight loss was calculated from the weight difference on day1 and day 8 and the colon length was measured from the body of the caecum to the distal part of the rectum. B The part of the colon was washed and IL-6 cytokine was measured using ELISA assay. C Blood was collected and IL-6 was detected in serum using ELISA. D-F Il6, Il1b, and <t>MyD88</t> mRNA expression was detected using qPCR in colon tissue homogenate. G <t>MyD88</t> <t>protein</t> detection from obtained colon tissue of treated mice, using Western blot. β-actin was used as loading control. H Schematic presentation of the experiment. LPS was i.p. injected. After 4 h IL-6 and IL-10 cytokines were analyzed in the serum. Combined data from one (B, C. H) or two (A, D-F) indep. exp. are shown as mean ± SEM. LN transformation was applied for the data used in statistical tests (D-F). Studentʾs unpaired t-test was used. p values of < 0.05 (*), < 0.01 (**) are indicated
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smta00  (Bio-Techne corporation)
97
Bio-Techne corporation smta00
Fig. 1 MyD88D162E mutant mice are protected from DSS-induced acute colitis. Schematic presentation of the DSS-induced colitis experiment. To induce acute colitis, mice were drinking tap water, supplemented with 2% DSS for 8 consecutive days ad libitum. A Disease activity index was defined by observing the stool consistency and the presence of the gross or occult blood. The weight loss was calculated from the weight difference on day1 and day 8 and the colon length was measured from the body of the caecum to the distal part of the rectum. B The part of the colon was washed and IL-6 cytokine was measured using ELISA assay. C Blood was collected and IL-6 was detected in serum using ELISA. D-F Il6, Il1b, and <t>MyD88</t> mRNA expression was detected using qPCR in colon tissue homogenate. G <t>MyD88</t> <t>protein</t> detection from obtained colon tissue of treated mice, using Western blot. β-actin was used as loading control. H Schematic presentation of the experiment. LPS was i.p. injected. After 4 h IL-6 and IL-10 cytokines were analyzed in the serum. Combined data from one (B, C. H) or two (A, D-F) indep. exp. are shown as mean ± SEM. LN transformation was applied for the data used in statistical tests (D-F). Studentʾs unpaired t-test was used. p values of < 0.05 (*), < 0.01 (**) are indicated
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pmje00  (Bio-Techne corporation)
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Bio-Techne corporation pmje00
Fig. 1 MyD88D162E mutant mice are protected from DSS-induced acute colitis. Schematic presentation of the DSS-induced colitis experiment. To induce acute colitis, mice were drinking tap water, supplemented with 2% DSS for 8 consecutive days ad libitum. A Disease activity index was defined by observing the stool consistency and the presence of the gross or occult blood. The weight loss was calculated from the weight difference on day1 and day 8 and the colon length was measured from the body of the caecum to the distal part of the rectum. B The part of the colon was washed and IL-6 cytokine was measured using ELISA assay. C Blood was collected and IL-6 was detected in serum using ELISA. D-F Il6, Il1b, and <t>MyD88</t> mRNA expression was detected using qPCR in colon tissue homogenate. G <t>MyD88</t> <t>protein</t> detection from obtained colon tissue of treated mice, using Western blot. β-actin was used as loading control. H Schematic presentation of the experiment. LPS was i.p. injected. After 4 h IL-6 and IL-10 cytokines were analyzed in the serum. Combined data from one (B, C. H) or two (A, D-F) indep. exp. are shown as mean ± SEM. LN transformation was applied for the data used in statistical tests (D-F). Studentʾs unpaired t-test was used. p values of < 0.05 (*), < 0.01 (**) are indicated
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pmta00  (Bio-Techne corporation)
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Bio-Techne corporation pmta00
Fig. 1 MyD88D162E mutant mice are protected from DSS-induced acute colitis. Schematic presentation of the DSS-induced colitis experiment. To induce acute colitis, mice were drinking tap water, supplemented with 2% DSS for 8 consecutive days ad libitum. A Disease activity index was defined by observing the stool consistency and the presence of the gross or occult blood. The weight loss was calculated from the weight difference on day1 and day 8 and the colon length was measured from the body of the caecum to the distal part of the rectum. B The part of the colon was washed and IL-6 cytokine was measured using ELISA assay. C Blood was collected and IL-6 was detected in serum using ELISA. D-F Il6, Il1b, and <t>MyD88</t> mRNA expression was detected using qPCR in colon tissue homogenate. G <t>MyD88</t> <t>protein</t> detection from obtained colon tissue of treated mice, using Western blot. β-actin was used as loading control. H Schematic presentation of the experiment. LPS was i.p. injected. After 4 h IL-6 and IL-10 cytokines were analyzed in the serum. Combined data from one (B, C. H) or two (A, D-F) indep. exp. are shown as mean ± SEM. LN transformation was applied for the data used in statistical tests (D-F). Studentʾs unpaired t-test was used. p values of < 0.05 (*), < 0.01 (**) are indicated
Pmta00, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pyrazolo[1,5-a]pyrimidine  (Enamine Ltd)
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Enamine Ltd pyrazolo[1,5-a]pyrimidine
Fig. 1 MyD88D162E mutant mice are protected from DSS-induced acute colitis. Schematic presentation of the DSS-induced colitis experiment. To induce acute colitis, mice were drinking tap water, supplemented with 2% DSS for 8 consecutive days ad libitum. A Disease activity index was defined by observing the stool consistency and the presence of the gross or occult blood. The weight loss was calculated from the weight difference on day1 and day 8 and the colon length was measured from the body of the caecum to the distal part of the rectum. B The part of the colon was washed and IL-6 cytokine was measured using ELISA assay. C Blood was collected and IL-6 was detected in serum using ELISA. D-F Il6, Il1b, and <t>MyD88</t> mRNA expression was detected using qPCR in colon tissue homogenate. G <t>MyD88</t> <t>protein</t> detection from obtained colon tissue of treated mice, using Western blot. β-actin was used as loading control. H Schematic presentation of the experiment. LPS was i.p. injected. After 4 h IL-6 and IL-10 cytokines were analyzed in the serum. Combined data from one (B, C. H) or two (A, D-F) indep. exp. are shown as mean ± SEM. LN transformation was applied for the data used in statistical tests (D-F). Studentʾs unpaired t-test was used. p values of < 0.05 (*), < 0.01 (**) are indicated
Pyrazolo[1,5 A]Pyrimidine, supplied by Enamine Ltd, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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smje00b  (Bio-Techne corporation)
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Bio-Techne corporation smje00b
Fig. 1 MyD88D162E mutant mice are protected from DSS-induced acute colitis. Schematic presentation of the DSS-induced colitis experiment. To induce acute colitis, mice were drinking tap water, supplemented with 2% DSS for 8 consecutive days ad libitum. A Disease activity index was defined by observing the stool consistency and the presence of the gross or occult blood. The weight loss was calculated from the weight difference on day1 and day 8 and the colon length was measured from the body of the caecum to the distal part of the rectum. B The part of the colon was washed and IL-6 cytokine was measured using ELISA assay. C Blood was collected and IL-6 was detected in serum using ELISA. D-F Il6, Il1b, and <t>MyD88</t> mRNA expression was detected using qPCR in colon tissue homogenate. G <t>MyD88</t> <t>protein</t> detection from obtained colon tissue of treated mice, using Western blot. β-actin was used as loading control. H Schematic presentation of the experiment. LPS was i.p. injected. After 4 h IL-6 and IL-10 cytokines were analyzed in the serum. Combined data from one (B, C. H) or two (A, D-F) indep. exp. are shown as mean ± SEM. LN transformation was applied for the data used in statistical tests (D-F). Studentʾs unpaired t-test was used. p values of < 0.05 (*), < 0.01 (**) are indicated
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butylated hydroxytoluene  (Enamine Ltd)
99
Enamine Ltd butylated hydroxytoluene
Fig. 1 MyD88D162E mutant mice are protected from DSS-induced acute colitis. Schematic presentation of the DSS-induced colitis experiment. To induce acute colitis, mice were drinking tap water, supplemented with 2% DSS for 8 consecutive days ad libitum. A Disease activity index was defined by observing the stool consistency and the presence of the gross or occult blood. The weight loss was calculated from the weight difference on day1 and day 8 and the colon length was measured from the body of the caecum to the distal part of the rectum. B The part of the colon was washed and IL-6 cytokine was measured using ELISA assay. C Blood was collected and IL-6 was detected in serum using ELISA. D-F Il6, Il1b, and <t>MyD88</t> mRNA expression was detected using qPCR in colon tissue homogenate. G <t>MyD88</t> <t>protein</t> detection from obtained colon tissue of treated mice, using Western blot. β-actin was used as loading control. H Schematic presentation of the experiment. LPS was i.p. injected. After 4 h IL-6 and IL-10 cytokines were analyzed in the serum. Combined data from one (B, C. H) or two (A, D-F) indep. exp. are shown as mean ± SEM. LN transformation was applied for the data used in statistical tests (D-F). Studentʾs unpaired t-test was used. p values of < 0.05 (*), < 0.01 (**) are indicated
Butylated Hydroxytoluene, supplied by Enamine Ltd, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sm6000b  (Bio-Techne corporation)
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Bio-Techne corporation sm6000b
Fig. 1 MyD88D162E mutant mice are protected from DSS-induced acute colitis. Schematic presentation of the DSS-induced colitis experiment. To induce acute colitis, mice were drinking tap water, supplemented with 2% DSS for 8 consecutive days ad libitum. A Disease activity index was defined by observing the stool consistency and the presence of the gross or occult blood. The weight loss was calculated from the weight difference on day1 and day 8 and the colon length was measured from the body of the caecum to the distal part of the rectum. B The part of the colon was washed and IL-6 cytokine was measured using ELISA assay. C Blood was collected and IL-6 was detected in serum using ELISA. D-F Il6, Il1b, and <t>MyD88</t> mRNA expression was detected using qPCR in colon tissue homogenate. G <t>MyD88</t> <t>protein</t> detection from obtained colon tissue of treated mice, using Western blot. β-actin was used as loading control. H Schematic presentation of the experiment. LPS was i.p. injected. After 4 h IL-6 and IL-10 cytokines were analyzed in the serum. Combined data from one (B, C. H) or two (A, D-F) indep. exp. are shown as mean ± SEM. LN transformation was applied for the data used in statistical tests (D-F). Studentʾs unpaired t-test was used. p values of < 0.05 (*), < 0.01 (**) are indicated
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smif00  (Bio-Techne corporation)
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Bio-Techne corporation smif00
Fig. 1 MyD88D162E mutant mice are protected from DSS-induced acute colitis. Schematic presentation of the DSS-induced colitis experiment. To induce acute colitis, mice were drinking tap water, supplemented with 2% DSS for 8 consecutive days ad libitum. A Disease activity index was defined by observing the stool consistency and the presence of the gross or occult blood. The weight loss was calculated from the weight difference on day1 and day 8 and the colon length was measured from the body of the caecum to the distal part of the rectum. B The part of the colon was washed and IL-6 cytokine was measured using ELISA assay. C Blood was collected and IL-6 was detected in serum using ELISA. D-F Il6, Il1b, and <t>MyD88</t> mRNA expression was detected using qPCR in colon tissue homogenate. G <t>MyD88</t> <t>protein</t> detection from obtained colon tissue of treated mice, using Western blot. β-actin was used as loading control. H Schematic presentation of the experiment. LPS was i.p. injected. After 4 h IL-6 and IL-10 cytokines were analyzed in the serum. Combined data from one (B, C. H) or two (A, D-F) indep. exp. are shown as mean ± SEM. LN transformation was applied for the data used in statistical tests (D-F). Studentʾs unpaired t-test was used. p values of < 0.05 (*), < 0.01 (**) are indicated
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human/mouse tnf-alpha antibody  (Bio-Techne corporation)
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Bio-Techne corporation human/mouse tnf-alpha antibody
Fig. 1 MyD88D162E mutant mice are protected from DSS-induced acute colitis. Schematic presentation of the DSS-induced colitis experiment. To induce acute colitis, mice were drinking tap water, supplemented with 2% DSS for 8 consecutive days ad libitum. A Disease activity index was defined by observing the stool consistency and the presence of the gross or occult blood. The weight loss was calculated from the weight difference on day1 and day 8 and the colon length was measured from the body of the caecum to the distal part of the rectum. B The part of the colon was washed and IL-6 cytokine was measured using ELISA assay. C Blood was collected and IL-6 was detected in serum using ELISA. D-F Il6, Il1b, and <t>MyD88</t> mRNA expression was detected using qPCR in colon tissue homogenate. G <t>MyD88</t> <t>protein</t> detection from obtained colon tissue of treated mice, using Western blot. β-actin was used as loading control. H Schematic presentation of the experiment. LPS was i.p. injected. After 4 h IL-6 and IL-10 cytokines were analyzed in the serum. Combined data from one (B, C. H) or two (A, D-F) indep. exp. are shown as mean ± SEM. LN transformation was applied for the data used in statistical tests (D-F). Studentʾs unpaired t-test was used. p values of < 0.05 (*), < 0.01 (**) are indicated
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Image Search Results


Fig. 1 MyD88D162E mutant mice are protected from DSS-induced acute colitis. Schematic presentation of the DSS-induced colitis experiment. To induce acute colitis, mice were drinking tap water, supplemented with 2% DSS for 8 consecutive days ad libitum. A Disease activity index was defined by observing the stool consistency and the presence of the gross or occult blood. The weight loss was calculated from the weight difference on day1 and day 8 and the colon length was measured from the body of the caecum to the distal part of the rectum. B The part of the colon was washed and IL-6 cytokine was measured using ELISA assay. C Blood was collected and IL-6 was detected in serum using ELISA. D-F Il6, Il1b, and MyD88 mRNA expression was detected using qPCR in colon tissue homogenate. G MyD88 protein detection from obtained colon tissue of treated mice, using Western blot. β-actin was used as loading control. H Schematic presentation of the experiment. LPS was i.p. injected. After 4 h IL-6 and IL-10 cytokines were analyzed in the serum. Combined data from one (B, C. H) or two (A, D-F) indep. exp. are shown as mean ± SEM. LN transformation was applied for the data used in statistical tests (D-F). Studentʾs unpaired t-test was used. p values of < 0.05 (*), < 0.01 (**) are indicated

Journal: Cell communication and signaling : CCS

Article Title: MyD88 protein destabilization mitigates NF-κB-dependent protection against macrophage apoptosis.

doi: 10.1186/s12964-024-01930-1

Figure Lengend Snippet: Fig. 1 MyD88D162E mutant mice are protected from DSS-induced acute colitis. Schematic presentation of the DSS-induced colitis experiment. To induce acute colitis, mice were drinking tap water, supplemented with 2% DSS for 8 consecutive days ad libitum. A Disease activity index was defined by observing the stool consistency and the presence of the gross or occult blood. The weight loss was calculated from the weight difference on day1 and day 8 and the colon length was measured from the body of the caecum to the distal part of the rectum. B The part of the colon was washed and IL-6 cytokine was measured using ELISA assay. C Blood was collected and IL-6 was detected in serum using ELISA. D-F Il6, Il1b, and MyD88 mRNA expression was detected using qPCR in colon tissue homogenate. G MyD88 protein detection from obtained colon tissue of treated mice, using Western blot. β-actin was used as loading control. H Schematic presentation of the experiment. LPS was i.p. injected. After 4 h IL-6 and IL-10 cytokines were analyzed in the serum. Combined data from one (B, C. H) or two (A, D-F) indep. exp. are shown as mean ± SEM. LN transformation was applied for the data used in statistical tests (D-F). Studentʾs unpaired t-test was used. p values of < 0.05 (*), < 0.01 (**) are indicated

Article Snippet: To determine the role of caspase-1 in MyD88 signaling regulation cells were transfected with pcDNA3.1 mouse MyD88wt (a gift from R. Medzhitov (Addgene plasmid #13,092; http:// n2t. net/ addge ne: 13092; RRID:Addgene_13092)) or pcDNA3.1 mouse MyD88D162E plasmids (both 0.05 ng/well) w/o increasing concentrations of pCMV mouse Caspase-1 (0.05, 0.1 ng/well) as well as pELAM1-luciferase (NF-κB promotor; 30 ng/well)) (a gift from C. Kirschning, Germany) and phRL-TK Renilla luciferase (3 ng/well) (Promega) for normalization using lipofectamine 2000 transfection reagent (Invitrogen).

Techniques: Mutagenesis, Activity Assay, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Control, Injection, Transformation Assay